The newest Thermo Scientific BioPharma Finder 5.1 software offers a solution for oligonucleotide analysis and biotherapeutic characterisation workflows, providing increased confidence in results by comprehensively characterising mass spectrometry data using advanced algorithms. Oligonucleotide-based biotherapeutics present unique challenges for characterisation due to their complexity. The new software provides an enhanced suite of built-for-purpose tools to overcome these challenges and meet the needs of this rapidly growing field, quickly and confidently turning complex data into meaningful results. Improvements to the kinetic prediction model for oligonucleotides enable more confident prediction and matching. Improved data review tools and tables offer streamlined review and results reporting of complex oligonucleotide data sets.