The latest Thermo Scientific Proteome Discoverer 3.0 software, in combination with the CHIMERYS intelligent search algorithm by MSAID, use artificial intelligence to more fully interpret data generated by Thermo Scientific Orbitrap mass spectrometers. This innovative approach substantially increases the number of unique peptide identifications, improves protein coverage and quantitation capabilities, and enables scientists to discover more in their data. The new intelligent search algorithm also facilitates higher throughput analysis and wide-isolation window data-dependent acquisition for synergistic performance enhancements. This deeper mining of data expands the ways in which proteomics scientists can acquire and apply their data.