SCIEX and Intabio have announced a collaboration to couple Intabio’s Blaze™ system with SCIEX high-resolution accurate mass spectrometers. This integration provides imaged capillary isoelectric focusing with mass spectrometry (iCIEF-MS) in order to simultaneously characterise and identify intact protein charge variants. The successful integration of iCIEF-MS workflows will streamline current laborious and time-consuming approaches in the biopharma environment to identify, monitor and characterise critical quality attributes in intact protein samples for the development and manufacture of next-gen therapeutics.
The commercial release of the Blaze™ system is scheduled for Q4 of 2020 and the integrated iCIEF-MS workflow will be available on SCIEX TripleTOF® 6600+ Systems.




![Targeted proton transfer charge reduction (tPTCR) nano-DESI mass spectrometry imaging of liver tissue from orally dosed rat (Animal 3). a) optical image of a blood vessel within liver tissue. b) Composite ion image of charge-reduced haeme-bound α-globin (7+ and 6+ charge states; m/z 2259.9 and m/z 2636.3 respectively, red) and the charged-reduced [FABP+bezafibrate] complex (7+ and 6+ charge states; m/z 2097.5 and m/z 2446.9 respectively, blue). c) Ion image composed from charge-reduced haeme-bound α-globin (7+ and 6+ charge states) showing abundance in blood vessels. d) Ion image composed from charge-reduced [FABP+bezafibrate] complex (7+ and 6+ charge states) showing abundance in bulk tissue and absence in the blood vessel. Reproduced from https://doi.org/10.1002/ange.202202075 under a CC BY licence. Light and mass spectromert imaging of tissue samples](/sites/default/files/styles/thumbnail/public/news/MSI%20drug-protein%20complex-w.jpg?itok=CBNIjyYl)




