Zeiss has recently developed and released a Handshake plug-in for their ZEN imaging software. This plug-in enables acquiring time-resolved data with PicoQuant’s LSM Upgrade Kit within the ZEN environment. This integration provides a way to optimise Fluorescence Lifetime Imaging (FLIM) or Fluorescence Correlation Spectroscopy (FCS) measurement workflow. The subsequent data analysis is performed employing the tools provided by PicoQuant’s SymPhoTime 64 software package. The Zeiss ZEN software integration allows setting up and controlling basic confocal, FLIM, FCS and FLCS measurements as well as more complex acquisition schemes such as multi-point FCS, time-series FLIM, or even 3D-FLIM images. All of these advanced acquisition modes can also be combined into a single experiment.
